![]() On day 6 and 13, 8 tumors were excised from rhodamine lectin-preinjected mice, permeabilized, stained for CD3 and imaged by LSFM. On day 6, 4 tumors were excised for cyclic IF where tumor sections were stained with a panel of 25 antibodies. ![]() CAR T cell distribution was measured in 2D BLI and 3D ♜T/BLT every 3-4 days. under the xenograft tumor on days 1, 3, 5 and 7 post-therapy-initiation at a dose of 25,000 IU/mouse. Therapeutic T cells were genetically modified to co-express the CAR of interest and the luciferase CBR2opt. Methods: NSG mice with subcutaneous AsPC1 xenograft tumors were treated with EGFR CAR T cell (± IL-2) or control BDCA-2 CAR T cell (± IL-2) (n = 7 each). We addressed this challenge by combining 3D ♜omputer tomography bioluminescence tomography (♜T/BLT), light-sheet fluorescence microscopy (LSFM) and cyclic immunofluorescence (IF) staining. Furthermore, a gap exists between macro- and microlevel imaging data to better assess intratumor infiltration of therapeutic cells. File import instruction AbstractĬAR T cell research in solid tumors often lacks spatiotemporal information and therefore, there is a need for a molecular tomography to facilitate high-throughput preclinical monitoring of CAR T cells. Select the file that you have just downloaded and select import option Reference Manager (RIS). A multimodal imaging workflow for monitoring CAR T cell therapy against solid tumor from whole-body to single-cell level. Pfeifer R, Henze J, Wittich K, Gosselink A, Kinkhabwala A, Gremse F, Bleilevens C, Bigott K, Jungblut M, Hardt O, Alves F, Al Rawashdeh W. ✉ Corresponding author: E-mail: wael.alrawashdehcom (W.A.). Ossium Health Inc, Indianapolis, Indiana, United States of America. ![]() Max-Planck-Institute for Multidisciplinary Science, Translational Molecular Imaging, Göttingen, Lower Saxony, Germany.Ħ. Gremse-IT GmbH, Aachen, North Rhine-Westphalia, Germany.ĥ. Institute of Medical Statistics and Computational Biology, University of Cologne, Cologne, North Rhine-Westphalia, Germany.Ĥ. University Medical Center Göttingen, Translational Molecular Imaging, Institute for Diagnostic and Interventional Radiology & Clinic for Haematology and Medical Oncology, Göttingen, Lower Saxony, Germany.ģ. KG, R&D Reagents, Bergisch Gladbach, North Rhine-Westphalia, Germany.Ģ. Rita Pfeifer 1*, Janina Henze 1,2*, Katharina Wittich 1, Andre Gosselink 1,3, Ali Kinkhabwala 1, Felix Gremse 4, Cathrin Bleilevens 1, Kevin Bigott 1, Melanie Jungblut 1, Olaf Hardt 1, Frauke Alves 2,5, Wa'el Al Rawashdeh 1,6ġ. Research Paper A multimodal imaging workflow for monitoring CAR T cell therapy against solid tumor from whole-body to single-cell level
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